Efecto de los medios KM y BBM usando luz led roja y azul sobre la producción de astaxantina en Haematococcus pluvialis
Autores/as
PDF
FLIP
HTML
Cómo citar
Torres Martínez , . N. L. ., Cuellar Bogotá, M. T. ., & Camacho Kurmen , J. E. . (2026). Efecto de los medios KM y BBM usando luz led roja y azul sobre la producción de astaxantina en Haematococcus pluvialis. Revista Mutis, 16(1), 1–21. https://doi.org/10.21789/22561498.2205
Más formatos de cita
Términos de licencia
▼
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.
Resumen
La optimización de la producción biotecnológica de astaxantina a partir de la microalga Haematococcus pluvialis Flotow (1848) (Chlamydomonadales, Chlorophyceae) cuando es sometida a condiciones de estrés, es importante por sus usos como pigmento y compuesto bioactivo en diferentes industrias. Es por esto por lo que se propuso como objetivo determinar el efecto de los medios km y bbm usando luz led roja (650 nm) con una irradiancia de 130.65 µmol/m2s y luz led azul (450 nm) con una irradiancia de 924.42 µmol/m2s sobre la producción de astaxantina en H. pluvialis. Se realizó el anova (95%) para establecer diferencias significativas entre tratamientos y prueba t.
Las condiciones de cultivo de la microalga H. pluvialis establecidas incluyen los medios km y bbm, que pueden utilizarse para su cultivo usando luces led, ya que no se presentaron diferencias significativas entre tratamientos (F:2.051; P:0.174; gl:1), siendo el medio de cultivo bbm, en el que se presentó el mayor crecimiento de la microalga llegando a 2,88x106 cel./mL, con una velocidad de crecimiento (R2=0,87, modelo logístico). En la producción de astaxantina, se presentaron diferencias significativas entre tratamientos (F:9.060; P:0.009; gl:1), encontrándose que el medio km, usando luces led (t=3,01; P=0.005), produjo mayor acumulación, 20,29 μg/mL, observándose morfológicamente quistes de tonalidad roja por su acumulación. Se concluye que el uso de medio de cultivo KM combinado con luz led roja (650 nm) con una irradiancia de 130.65 µmol/m2s y luz led azul (450 nm) con una irradiancia de 924.42 µmol/m2s incrementó la acumulación de astaxantina.
Citas
Achaempong, A., Li, L., Elsherbiny, S. M., Wu, Y., Swallah, M. S., Bondzie-Quaye, P., & Huang, Q. (2024). A crosswalk on the genetic and conventional strategies for enhancing astaxanthin production in Haematococcus pluvialis. Critical Reviews in Biotechnology, 44(6), 1018-1039. https://doi.org/10.1080/07388551.2023.2240009
American Public Health Association. (2005). Standard methods for the examination of water and wastewater (21st ed.). American Public Health Association.
Busakorn, W., Chan, S. S., Ling, T. C., Show, P. L., Ng, E. P., Woranart, J., et al. (2023). Advancement of carotenogenesis of astaxanthin from Haematococcus pluvialis: Recent insight and way forward. Molecular Biotechnology, 66(3), 402–423. https://doi.org/10.1007/s12033-023-00768-1
Camacho Kurmen, J. E., & Rodríguez Rodríguez, N. (2024). Producción de astaxantina en Haematococcus pluvialis bajo el efecto de la deficiencia de fosfatos y alta intensidad de luz. Revista ION, 37(2), 49–64. https://doi.org/10.18273/revion.v37n2-2024004
Casula, M., Fais, G., Manis, C., Scano, P., Concas, A., Cao, G., et al. (2024). The production of FAHFA is enhanced when Haematococcus pluvialis is grown in CO₂. Food Chemistry, 449, 139165. https://doi.org/10.1016/j.foodchem.2024.139165
Cheirsilp, B., Wantip, K., Chai-issarapap, N., Maneechote, W., Pekkoh, J., Duangjan, K., et al. (2022). Enhanced production of astaxanthin and co-bioproducts from microalga Haematococcus sp. integrated with valorization of industrial wastewater under two-stage LED light illumination strategy. Environmental Technology & Innovation, 28, 102620. https://doi.org/10.1016/j.eti.2022.102620
Christian, D., Zhang, J., Sawdon, A. J., & Peng, C. A. (2018). Enhanced astaxanthin accumulation in Haematococcus pluvialis using high carbon dioxide concentration and light illumination. Bioresource Technology, 256, 548–551. https://doi.org/10.1016/j.biortech.2018.02.074
De Moraes, L. B. S., Mota, G. C. P., Molina-Miras, A., Sánchez-Mirón, A., Cerón-García, M. D. C., Olivera Gálvez, A., et al. (2025). Enhancing high-value metabolite production and asparaginase activity in Haematococcus pluvialis: impact of different organic carbon sources and feeding strategies. Biomass Conversion and Biorefinery, 1-14. https://doi.org/10.1007/s13399-025-06779-z
Do, T. T., Quach-Van, T. E., Nguyen, T. C., Show, P. L., Nguyen, T. M. L., Huynh, D. H., et al. (2023). Effect of LED illumination cycle and carbon sources on biofilms of Haematococcus pluvialis in pilot-scale angLED twin-layer porous substrate photobioreactors. Bioengineering, 10(5), 596. https://doi.org/10.3390/bioengineering10050596
Do, T. T., Tran-Thi, B. H., Ong, B. N., Le, T. L., Nguyen, T. C., Quan, Q. D., et al. (2021). Effects of red and blue light emitting diodes on biomass and astaxanthin of Haematococcus pluvialis in pilot scale angLED twin-layer porous substrate photobioreactors. Life Sciences | Biotechnology, 2020(3–5). https://doi.org/10.3390/biology8030068
Gao, Z., Meng, C., Li, Y., Liu, L., Deng, S., Shen, Y., et al. (2014). Impact of blue light from LED on astaxanthin accumulation and transcription of key carotenogenetics in Haematococcus pluvialis. Algal Research, 8(5), 1–9.
Gómez, L., Orozco, M. I., Quiroga, C., Díaz, J. C., & Huérfano, J. (2019). Producción de astaxantina y expresión de genes en Haematococcus pluvialis (Chlorophyceae, Volvocales) bajo condiciones de estrés por deficiencia de nitrógeno y alta irradiancia. Revista Mutis, 9(2), 7–24. https://doi.org/10.21789/22561498.1532
Hazwani, N., Shoparwe, N., Yusoff, A. H., Rahim, A. A., Chang, C. S., Tan, J. S., et al. (2021). A review on Haematococcus pluvialis bioprocess optimization of green and red stage culture conditions for the production of natural astaxanthin. Biomolecules, 11(2), 256. https://doi.org/10.3390/biom11020256
He, B., et al. (2020). Ultrastructural changes of Haematococcus pluvialis (Chlorophyta) in process of astaxanthin accumulation and cell damage under condition of high light with acetate. Algae, 35(3), 253–262. https://doi.org/10.4490/algae.2020.35.5.22
Herrera, A. D. G., Martínez, I. M. G., & Kurmen, J. E. C. (2024). Revisión: efecto del factor estrés, color e intensidad de luz sobre la producción de astaxantina en Haematococcus pluvialis. Revista Mutis, 14(2), 1-33. https://doi.org/10.21789/22561498.2108
Hu, Q., Hu, Z., Yan, X., Lu, J., & Wang, C. (2024). Extracellular vesicles involved in growth regulation and metabolic modulation in Haematococcus pluvialis. Biotechnology for Biofuels and Bioproducts, 17(1), 24. https://doi.org/10.1186/s13068-024-02462-z
İmamoğlu, E. (2007). Effect of different culture media and light intensities on growth of Haematococcus pluvialis. International Journal of Natural and Engineering Sciences, 1(3), 5–9.
Jin, C., Zhou, Z., Zhu, Y., et al. (2024). Photosynthetic activity and astaxanthin production of Haematococcus pluvialis regulated by manipulated light quality. Aquaculture International, 32, 3617–3635. https://doi.org/10.1007/s10499-023-01341-y
Katsuda, T., Lababpur, S., Shimahara, K., & Katoh, S. (2004). Astaxanthin production by Haematococcus pluvialis under illumination with LEDs. Biotechnology Advances, 35(1), 107602. https://doi.org/10.1016/j.biotechadv.2020.107602
Khoo, K. S., Lee, S. Y., Ooi, C. W., Fu, X., Miao, X., Ling, T. C., et al. (2019). Recent advances in biorefinery of astaxanthin from Haematococcus pluvialis. Bioresource Technology, 288, 121606. https://doi.org/10.1016/j.biortech.2019.121606
Kirsch, B. J., Bennun, S. V., Mendez, A., Johnson, A. S., Wang, H., Qiu, H., Li, N., Lawrence, S. M., Bak, H., & Betenbaugh, M. J. (2022). Metabolic analysis of the asparagine and glutamine dynamics in an industrial Chinese hamster ovary fed‐batch process. Biotechnology and Bioengineering, 119(3), 807–819. https://doi.org/10.1002/bit.27993
Kobayashi, M., Kakizono, T., & Nagai, S. (1993). Enhanced carotenoid biosynthesis by oxidative stress in acetate-induced cyst cells of a green unicellular alga, Haematococcus pluvialis. Applied and Environmental Microbiology, 59(3), 867-873. https://doi.org/10.1128/aem.59.3.867-873.1993
Lee, J., Ahn, Y., Kim, J., Kim, Y., & Choi, Y. (2018). Comparative transcriptome analysis of Haematococcus pluvialis on astaxanthin biosynthesis in response to irradiation with red or blue light. World Journal of Microbiology and Biotechnology, 34(96). https://doi.org/10.1007/s11274-018-2459-y
Li, F., Zhang, N., Zhang, Y., Lian, Q., Qin, C., Qian, Z., et al. (2021). NaCl promotes the efficient formation of Haematococcus pluvialis nonmotile cells under phosphorus deficiency. Marine Drugs, 19(6), 337. https://doi.org/10.3390/md19060337
Li, K., Ye, Q., Li, Q., Xia, R., Guo, W., & Cheng, J. (2020). Effects of the spatial and spectral distribution of red and blue light on Haematococcus pluvialis growth. Algal Research, 51, 102045. https://doi.org/10.1016/j.algal.2020.102045
Li, X., Wang, X., Duan, S., Yi, S., Gao, Z., Xiao, C., et al. (2020). Biotechnological production of astaxanthin from the microalga Haematococcus pluvialis. Biotechnology Advances, 43, 107602. https://doi.org/10.1016/j.biotechadv.2020.107602
Liyanaarachchi, V. C., Nishshanka, G. K. S. H., Premaratne, R. G. M. M., Ariyadasa, T. U., Nimarshana, P. H. V., & Malik, A. (2020). Astaxanthin accumulation in the green microalga Haematococcus pluvialis: Effect of initial phosphate concentration and stepwise/continuous light stress. Biotechnology Reports, 28, e00538. https://doi.org/10.1016/j.btre.2020.e00538
Ma, R., Thomas-Hall, S. R., Chua, E. T., Eltanahy, E., Netzel, M. E., Netzel, G., et al. (2018). Blue light enhances astaxanthin biosynthesis metabolism and extraction efficiency in Haematococcus pluvialis by inducing haematocyst germination. Algal Research, 35, 215–222. https://doi.org/10.1016/j.algal.2018.08.023
Marinho, Y. F., Malafaia, C. B., de Araújo, K. S., et al. (2021). Evaluation of the influence of different culture media on growth, life cycle, biochemical composition, and astaxanthin production in Haematococcus pluvialis. Aquaculture International, 29, 757–778. https://doi.org/10.1007/s10499-021-00655-z
Mazumdar, N., Novis, P., Visnovsky, G., & Gostomski, P. (2019). Effect of culturing parameters on the vegetative growth of Haematococcus alpinus (strain LCR-CC-261F) and modeling of its growth kinetics. Journal of Phycology, 55, 1071–1081. https://doi.org/10.1111/jpy.12894
Miranda, A., Ossa, E., Vargas, G., & Sáez, A. (2019). Efecto de las bajas concentraciones de nitratos y fosfatos sobre la acumulación de astaxantina en Haematococcus pluvialis UTEX 2505. Información Tecnológica, 30(1), 25–34. https://doi.org/10.4067/S0718-07642019000100023
Mota, G. C. P., Moraes, L. B. S., Oliveira, C. Y. B., Oliveira, D. W. S., Abreu, J. L., Dantas, D. M. M., et al. (2022). Astaxanthin from Haematococcus pluvialis: Processes, applications, and market. Preparative Biochemistry & Biotechnology, 52(5), 598–609. https://doi.org/10.1080/10826068.2021.1966802
Mourya, M., Khan, M. J., Sirotiya, V., Ahirwar, A., Schoefs, B., Marchand, J., Varjani, S., & Vinayak, V. (2023). Enhancing the biochemical growth of Haematococcus pluvialis by mitigation of broad-spectrum light stress in wastewater cultures. RSC Advances, 13(26), 17611–17620. https://doi.org/10.1039/d3ra01530k
Mularczyk, M., Michalak, I., & Marycz, K. (2020). Astaxanthin and other nutrients from Haematococcus pluvialis—Multifunctional applications. Marine Drugs, 18(9), 459. https://doi.org/10.3390/md18090459
Nemani, N., Dehnavi, S. M., & Pazuki, G. (2024). Extraction and separation of astaxanthin with the help of pre-treatment of Haematococcus pluvialis microalgae biomass using aqueous two-phase systems based on deep eutectic solvents. Scientific Reports, 14, 5420. https://doi.org/10.1038/s41598-024-55630-4
Ortiz-Moreno, M. L., Cárdenas-Poblador, J., Agredo, J., & Solarte-Murillo, L. V. (2020). Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum. Universitas Scientiarum, 25(1), 113–148. https://doi.org/10.11144/Javeriana.SC25-1.mteo
Oslan, S. N. H., Shoparwe, N. F., Yusoff, A. H., Rahim, A. A., Chang, C. S., Tan, J. S., et al. (2021). A review on Haematococcus pluvialis bioprocess optimization of green and red stage culture conditions for the production of natural astaxanthin. Biomolecules, 11(2), 256. https://doi.org/10.3390/biom11020256
Ota, S., Morita, A., Ohnuki, S., et al. (2018). Dinámica de carotenoides y gotas lipídicas que contienen astaxantina en respuesta a la luz en el alga verde Haematococcus pluvialis. Scientific Reports, 8, 5617. https://doi.org/10.1038/s41598-018-23854-w
Pang, N., Fu, X., Martinez, J. S., & Chen, S. (2019). Multilevel heuristic LED regime for stimulating lipid and bioproducts biosynthesis in Haematococcus pluvialis under mixotrophic conditions. Bioresource Technology, 288, 121525. https://doi.org/10.1016/j.biortech.2019.121525
Pereira, S., & Otero, A. (2020). Haematococcus pluvialis bioprocess optimization: Effect of light quality, temperature and irradiance on growth, pigment content and photosynthetic activity. Algal Research, 51, 102027. https://doi.org/10.1016/j.algal.2020.102027
Pham, K. T., Nguyen, T. C., Luong, T. H., Dang, P. H., Vu, D. C., Do, T. N., et al. (2018). Influence of inoculum size, CO₂ concentration and LEDs on the growth of green microalgae Haematococcus pluvialis Flotow. Vietnam Journal of Science, Technology and Engineering, 60(4), 59–65. https://doi.org/10.31276/VJSTE.60(4).59-65
Shah, M., Liang, Y., Cheng, J., & Daroch, M. (2016). Astaxanthin-producing green microalga Haematococcus pluvialis: From single cell to high-value commercial products. Frontiers in Plant Science, 7, 531. https://doi.org/10.3389/fpls.2016.00531
Rayamajhi, V., Byeon, H., An, Y., Kim, T., Lee, J., Lee, J., ... & Jung, S. (2025). Enhanced production of astaxanthin, chlorophyll, and biomass in Haematococcus lacustris (Chlorophyta) using inorganic and organic carbon sources. Phycologia, 1-11. https://doi.org/10.1080/00318884.2025.2577083
Rao, A. R., Siew, M., Ravi, S., & Gokare, A. (2014). Astaxanthin: Sources, extraction, stability, biological activities and its commercial applications—A review. Marine Drugs, 12(1), 128–152. https://doi.org/10.3390/md12010128
Ren, Y., Deng, J., Huang, J., Wu, Z., Yi, L., Bi, Y., et al. (2021). Using green alga Haematococcus pluvialis for astaxanthin and lipid co-production: Advances and outlook. Bioresource Technology, 340, 125736. https://doi.org/10.1016/j.biortech.2021.125736
Rodríguez-Amaya, D. B., Esquivel, P., & Meléndez-Martínez, A. J. (2023). Actualización integral sobre colorantes carotenoides provenientes de plantas y microalgas: Retos y avances desde los laboratorios de investigación hasta la industria. Foods, 12(22), 4080. https://doi.org/10.3390/foods12224080
Samhat, K., Kazbar, A., Takache, H., Ismail, A., & Pruvost, J. (2023). Influence of light absorption rate on the astaxanthin production by the microalga Haematococcus pluvialis during nitrogen starvation. Bioresources and Bioprocessing, 10(1), 70. https://doi.org/10.1186/s40643-023-00700-0
Santos, B., da Conceição, D. P., Corrêa, D. O., et al. (2022). Changes in gene expression and biochemical composition of Haematococcus pluvialis grown under different light colors. Journal of Applied Phycology, 34, 729–743. https://doi.org/10.1007/s10811-022-02696-0
Scibilia, L., Girolomoni, L., Berteotti, S., Alboresi, A., & Ballottari, M. (2015). Photosynthetic response to nitrogen starvation and high light in Haematococcus pluvialis. Algal Research, 12, 170–181. https://doi.org/10.1016/j.algal.2015.08.024
Silva, D. L., de Moraes, L. B. S., Oliveira, C. Y. B., da Silva Campos, C. V. F., de Souza Bezerra, R., & Gálvez, A. O. (2022). Influence of culture medium on growth and protein production by Haematococcus pluvialis. Acta Scientiarum. Technology, 44, e59590. https://doi.org/10.4025/actascitechnol.v44i1.59590
Sun, H., Liu, B., Lu, X., Cheng, K. W., & Chen, F. (2017). Staged cultivation enhances biomass accumulation in the green growth phase of Haematococcus pluvialis. Bioresource Technology, 233, 326–331. https://doi.org/10.1016/j.biortech.2017.03.011
Tocquin, P., Fratamico, A., & Franck, F. (2012). Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of a low N/P ratio on vegetative growth. Journal of Applied Phycology, 24(3), 365–373. https://doi.org/10.1007/s10811-011-9771-3
Tran, H. L., Lee, K. H., & Hong, C. H. (2015). Efectos de la irradiación con LED en el crecimiento y la producción de astaxantina de Haematococcus lacustris. Biosciences Biotechnology Research Asia, 12(2), 1153–1159. https://doi.org/10.13005/bbra/1778
Wang, Q., Wang, H., Ning, Y., Luo, Q., Yang, X., Huang, Y., et al. (2021). Transcription factors from Haematococcus pluvialis involved in the regulation of astaxanthin biosynthesis under high light–sodium acetate stress. Frontiers in Bioengineering and Biotechnology, 9, 2021. https://doi.org/10.3389/fbioe.2021.650178
Wang, J., Sommerfeld, M. R., Lu, C., & Hu, Q. (2013). Combined effect of initial biomass density and nitrogen concentration on growth and astaxanthin production of Haematococcus pluvialis (Chlorophyta) in outdoor cultivation. Algae, 28(2), 193–202. https://doi.org/10.4490/algae.2013.28.2.193
Wei, Z., Sun, F., Meng, C., Xing, W., Zhu, X., Wang, C., et al. (2022). Transcriptome analysis of the accumulation of astaxanthin in Haematococcus pluvialis treated with white and blue lights as well as salicylic acid. BioMed Research International, 2022, 4827595. https://doi.org/10.1155/2022/4827595
Xi, T., Kim, D. G., Roh, W., Choi, J. S., & Choi, Y. E. (2016). Enhancement of astaxanthin production using Haematococcus pluvialis with novel LED wavelength shift strategy. Applied Microbiology and Biotechnology, 100, 6231–6238. https://doi.org/10.1007/s00253-016-7301-6
Zhang, L., Su, F., Zhang, C., Gong, F., & Liu, J. (2016). Changes of photosynthetic behaviors and photoprotection during cell transformation and astaxanthin accumulation in Haematococcus pluvialis grown outdoors in tubular photobioreactors. International Journal of Molecular Sciences, 18(1), 33. https://doi.org/10.3390/ijms18010033
Zhang, W., Zhou, X., Zhang, Y., Cheng, P., Ma, R., Cheng, W., et al. (2018). Enhancing astaxanthin accumulation in Haematococcus pluvialis by coupLED light intensity and nitrogen starvation in column photobioreactors. Journal of Microbiology and Biotechnology, 28(12), 2019–2028. https://doi.org/10.4014/jmb.1807.07008
Zhao, Y., Yue, C., Geng, S., Ning, D., Ma, T., & Yu, X. (2019). Role of media composition in biomass and astaxanthin production of Haematococcus pluvialis under two-stage cultivation. Bioprocess and Biosystems Engineering, 42, 1–3. https://doi.org/10.1007/s00449-018-02064-8
American Public Health Association. (2005). Standard methods for the examination of water and wastewater (21st ed.). American Public Health Association.
Busakorn, W., Chan, S. S., Ling, T. C., Show, P. L., Ng, E. P., Woranart, J., et al. (2023). Advancement of carotenogenesis of astaxanthin from Haematococcus pluvialis: Recent insight and way forward. Molecular Biotechnology, 66(3), 402–423. https://doi.org/10.1007/s12033-023-00768-1
Camacho Kurmen, J. E., & Rodríguez Rodríguez, N. (2024). Producción de astaxantina en Haematococcus pluvialis bajo el efecto de la deficiencia de fosfatos y alta intensidad de luz. Revista ION, 37(2), 49–64. https://doi.org/10.18273/revion.v37n2-2024004
Casula, M., Fais, G., Manis, C., Scano, P., Concas, A., Cao, G., et al. (2024). The production of FAHFA is enhanced when Haematococcus pluvialis is grown in CO₂. Food Chemistry, 449, 139165. https://doi.org/10.1016/j.foodchem.2024.139165
Cheirsilp, B., Wantip, K., Chai-issarapap, N., Maneechote, W., Pekkoh, J., Duangjan, K., et al. (2022). Enhanced production of astaxanthin and co-bioproducts from microalga Haematococcus sp. integrated with valorization of industrial wastewater under two-stage LED light illumination strategy. Environmental Technology & Innovation, 28, 102620. https://doi.org/10.1016/j.eti.2022.102620
Christian, D., Zhang, J., Sawdon, A. J., & Peng, C. A. (2018). Enhanced astaxanthin accumulation in Haematococcus pluvialis using high carbon dioxide concentration and light illumination. Bioresource Technology, 256, 548–551. https://doi.org/10.1016/j.biortech.2018.02.074
De Moraes, L. B. S., Mota, G. C. P., Molina-Miras, A., Sánchez-Mirón, A., Cerón-García, M. D. C., Olivera Gálvez, A., et al. (2025). Enhancing high-value metabolite production and asparaginase activity in Haematococcus pluvialis: impact of different organic carbon sources and feeding strategies. Biomass Conversion and Biorefinery, 1-14. https://doi.org/10.1007/s13399-025-06779-z
Do, T. T., Quach-Van, T. E., Nguyen, T. C., Show, P. L., Nguyen, T. M. L., Huynh, D. H., et al. (2023). Effect of LED illumination cycle and carbon sources on biofilms of Haematococcus pluvialis in pilot-scale angLED twin-layer porous substrate photobioreactors. Bioengineering, 10(5), 596. https://doi.org/10.3390/bioengineering10050596
Do, T. T., Tran-Thi, B. H., Ong, B. N., Le, T. L., Nguyen, T. C., Quan, Q. D., et al. (2021). Effects of red and blue light emitting diodes on biomass and astaxanthin of Haematococcus pluvialis in pilot scale angLED twin-layer porous substrate photobioreactors. Life Sciences | Biotechnology, 2020(3–5). https://doi.org/10.3390/biology8030068
Gao, Z., Meng, C., Li, Y., Liu, L., Deng, S., Shen, Y., et al. (2014). Impact of blue light from LED on astaxanthin accumulation and transcription of key carotenogenetics in Haematococcus pluvialis. Algal Research, 8(5), 1–9.
Gómez, L., Orozco, M. I., Quiroga, C., Díaz, J. C., & Huérfano, J. (2019). Producción de astaxantina y expresión de genes en Haematococcus pluvialis (Chlorophyceae, Volvocales) bajo condiciones de estrés por deficiencia de nitrógeno y alta irradiancia. Revista Mutis, 9(2), 7–24. https://doi.org/10.21789/22561498.1532
Hazwani, N., Shoparwe, N., Yusoff, A. H., Rahim, A. A., Chang, C. S., Tan, J. S., et al. (2021). A review on Haematococcus pluvialis bioprocess optimization of green and red stage culture conditions for the production of natural astaxanthin. Biomolecules, 11(2), 256. https://doi.org/10.3390/biom11020256
He, B., et al. (2020). Ultrastructural changes of Haematococcus pluvialis (Chlorophyta) in process of astaxanthin accumulation and cell damage under condition of high light with acetate. Algae, 35(3), 253–262. https://doi.org/10.4490/algae.2020.35.5.22
Herrera, A. D. G., Martínez, I. M. G., & Kurmen, J. E. C. (2024). Revisión: efecto del factor estrés, color e intensidad de luz sobre la producción de astaxantina en Haematococcus pluvialis. Revista Mutis, 14(2), 1-33. https://doi.org/10.21789/22561498.2108
Hu, Q., Hu, Z., Yan, X., Lu, J., & Wang, C. (2024). Extracellular vesicles involved in growth regulation and metabolic modulation in Haematococcus pluvialis. Biotechnology for Biofuels and Bioproducts, 17(1), 24. https://doi.org/10.1186/s13068-024-02462-z
İmamoğlu, E. (2007). Effect of different culture media and light intensities on growth of Haematococcus pluvialis. International Journal of Natural and Engineering Sciences, 1(3), 5–9.
Jin, C., Zhou, Z., Zhu, Y., et al. (2024). Photosynthetic activity and astaxanthin production of Haematococcus pluvialis regulated by manipulated light quality. Aquaculture International, 32, 3617–3635. https://doi.org/10.1007/s10499-023-01341-y
Katsuda, T., Lababpur, S., Shimahara, K., & Katoh, S. (2004). Astaxanthin production by Haematococcus pluvialis under illumination with LEDs. Biotechnology Advances, 35(1), 107602. https://doi.org/10.1016/j.biotechadv.2020.107602
Khoo, K. S., Lee, S. Y., Ooi, C. W., Fu, X., Miao, X., Ling, T. C., et al. (2019). Recent advances in biorefinery of astaxanthin from Haematococcus pluvialis. Bioresource Technology, 288, 121606. https://doi.org/10.1016/j.biortech.2019.121606
Kirsch, B. J., Bennun, S. V., Mendez, A., Johnson, A. S., Wang, H., Qiu, H., Li, N., Lawrence, S. M., Bak, H., & Betenbaugh, M. J. (2022). Metabolic analysis of the asparagine and glutamine dynamics in an industrial Chinese hamster ovary fed‐batch process. Biotechnology and Bioengineering, 119(3), 807–819. https://doi.org/10.1002/bit.27993
Kobayashi, M., Kakizono, T., & Nagai, S. (1993). Enhanced carotenoid biosynthesis by oxidative stress in acetate-induced cyst cells of a green unicellular alga, Haematococcus pluvialis. Applied and Environmental Microbiology, 59(3), 867-873. https://doi.org/10.1128/aem.59.3.867-873.1993
Lee, J., Ahn, Y., Kim, J., Kim, Y., & Choi, Y. (2018). Comparative transcriptome analysis of Haematococcus pluvialis on astaxanthin biosynthesis in response to irradiation with red or blue light. World Journal of Microbiology and Biotechnology, 34(96). https://doi.org/10.1007/s11274-018-2459-y
Li, F., Zhang, N., Zhang, Y., Lian, Q., Qin, C., Qian, Z., et al. (2021). NaCl promotes the efficient formation of Haematococcus pluvialis nonmotile cells under phosphorus deficiency. Marine Drugs, 19(6), 337. https://doi.org/10.3390/md19060337
Li, K., Ye, Q., Li, Q., Xia, R., Guo, W., & Cheng, J. (2020). Effects of the spatial and spectral distribution of red and blue light on Haematococcus pluvialis growth. Algal Research, 51, 102045. https://doi.org/10.1016/j.algal.2020.102045
Li, X., Wang, X., Duan, S., Yi, S., Gao, Z., Xiao, C., et al. (2020). Biotechnological production of astaxanthin from the microalga Haematococcus pluvialis. Biotechnology Advances, 43, 107602. https://doi.org/10.1016/j.biotechadv.2020.107602
Liyanaarachchi, V. C., Nishshanka, G. K. S. H., Premaratne, R. G. M. M., Ariyadasa, T. U., Nimarshana, P. H. V., & Malik, A. (2020). Astaxanthin accumulation in the green microalga Haematococcus pluvialis: Effect of initial phosphate concentration and stepwise/continuous light stress. Biotechnology Reports, 28, e00538. https://doi.org/10.1016/j.btre.2020.e00538
Ma, R., Thomas-Hall, S. R., Chua, E. T., Eltanahy, E., Netzel, M. E., Netzel, G., et al. (2018). Blue light enhances astaxanthin biosynthesis metabolism and extraction efficiency in Haematococcus pluvialis by inducing haematocyst germination. Algal Research, 35, 215–222. https://doi.org/10.1016/j.algal.2018.08.023
Marinho, Y. F., Malafaia, C. B., de Araújo, K. S., et al. (2021). Evaluation of the influence of different culture media on growth, life cycle, biochemical composition, and astaxanthin production in Haematococcus pluvialis. Aquaculture International, 29, 757–778. https://doi.org/10.1007/s10499-021-00655-z
Mazumdar, N., Novis, P., Visnovsky, G., & Gostomski, P. (2019). Effect of culturing parameters on the vegetative growth of Haematococcus alpinus (strain LCR-CC-261F) and modeling of its growth kinetics. Journal of Phycology, 55, 1071–1081. https://doi.org/10.1111/jpy.12894
Miranda, A., Ossa, E., Vargas, G., & Sáez, A. (2019). Efecto de las bajas concentraciones de nitratos y fosfatos sobre la acumulación de astaxantina en Haematococcus pluvialis UTEX 2505. Información Tecnológica, 30(1), 25–34. https://doi.org/10.4067/S0718-07642019000100023
Mota, G. C. P., Moraes, L. B. S., Oliveira, C. Y. B., Oliveira, D. W. S., Abreu, J. L., Dantas, D. M. M., et al. (2022). Astaxanthin from Haematococcus pluvialis: Processes, applications, and market. Preparative Biochemistry & Biotechnology, 52(5), 598–609. https://doi.org/10.1080/10826068.2021.1966802
Mourya, M., Khan, M. J., Sirotiya, V., Ahirwar, A., Schoefs, B., Marchand, J., Varjani, S., & Vinayak, V. (2023). Enhancing the biochemical growth of Haematococcus pluvialis by mitigation of broad-spectrum light stress in wastewater cultures. RSC Advances, 13(26), 17611–17620. https://doi.org/10.1039/d3ra01530k
Mularczyk, M., Michalak, I., & Marycz, K. (2020). Astaxanthin and other nutrients from Haematococcus pluvialis—Multifunctional applications. Marine Drugs, 18(9), 459. https://doi.org/10.3390/md18090459
Nemani, N., Dehnavi, S. M., & Pazuki, G. (2024). Extraction and separation of astaxanthin with the help of pre-treatment of Haematococcus pluvialis microalgae biomass using aqueous two-phase systems based on deep eutectic solvents. Scientific Reports, 14, 5420. https://doi.org/10.1038/s41598-024-55630-4
Ortiz-Moreno, M. L., Cárdenas-Poblador, J., Agredo, J., & Solarte-Murillo, L. V. (2020). Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum. Universitas Scientiarum, 25(1), 113–148. https://doi.org/10.11144/Javeriana.SC25-1.mteo
Oslan, S. N. H., Shoparwe, N. F., Yusoff, A. H., Rahim, A. A., Chang, C. S., Tan, J. S., et al. (2021). A review on Haematococcus pluvialis bioprocess optimization of green and red stage culture conditions for the production of natural astaxanthin. Biomolecules, 11(2), 256. https://doi.org/10.3390/biom11020256
Ota, S., Morita, A., Ohnuki, S., et al. (2018). Dinámica de carotenoides y gotas lipídicas que contienen astaxantina en respuesta a la luz en el alga verde Haematococcus pluvialis. Scientific Reports, 8, 5617. https://doi.org/10.1038/s41598-018-23854-w
Pang, N., Fu, X., Martinez, J. S., & Chen, S. (2019). Multilevel heuristic LED regime for stimulating lipid and bioproducts biosynthesis in Haematococcus pluvialis under mixotrophic conditions. Bioresource Technology, 288, 121525. https://doi.org/10.1016/j.biortech.2019.121525
Pereira, S., & Otero, A. (2020). Haematococcus pluvialis bioprocess optimization: Effect of light quality, temperature and irradiance on growth, pigment content and photosynthetic activity. Algal Research, 51, 102027. https://doi.org/10.1016/j.algal.2020.102027
Pham, K. T., Nguyen, T. C., Luong, T. H., Dang, P. H., Vu, D. C., Do, T. N., et al. (2018). Influence of inoculum size, CO₂ concentration and LEDs on the growth of green microalgae Haematococcus pluvialis Flotow. Vietnam Journal of Science, Technology and Engineering, 60(4), 59–65. https://doi.org/10.31276/VJSTE.60(4).59-65
Shah, M., Liang, Y., Cheng, J., & Daroch, M. (2016). Astaxanthin-producing green microalga Haematococcus pluvialis: From single cell to high-value commercial products. Frontiers in Plant Science, 7, 531. https://doi.org/10.3389/fpls.2016.00531
Rayamajhi, V., Byeon, H., An, Y., Kim, T., Lee, J., Lee, J., ... & Jung, S. (2025). Enhanced production of astaxanthin, chlorophyll, and biomass in Haematococcus lacustris (Chlorophyta) using inorganic and organic carbon sources. Phycologia, 1-11. https://doi.org/10.1080/00318884.2025.2577083
Rao, A. R., Siew, M., Ravi, S., & Gokare, A. (2014). Astaxanthin: Sources, extraction, stability, biological activities and its commercial applications—A review. Marine Drugs, 12(1), 128–152. https://doi.org/10.3390/md12010128
Ren, Y., Deng, J., Huang, J., Wu, Z., Yi, L., Bi, Y., et al. (2021). Using green alga Haematococcus pluvialis for astaxanthin and lipid co-production: Advances and outlook. Bioresource Technology, 340, 125736. https://doi.org/10.1016/j.biortech.2021.125736
Rodríguez-Amaya, D. B., Esquivel, P., & Meléndez-Martínez, A. J. (2023). Actualización integral sobre colorantes carotenoides provenientes de plantas y microalgas: Retos y avances desde los laboratorios de investigación hasta la industria. Foods, 12(22), 4080. https://doi.org/10.3390/foods12224080
Samhat, K., Kazbar, A., Takache, H., Ismail, A., & Pruvost, J. (2023). Influence of light absorption rate on the astaxanthin production by the microalga Haematococcus pluvialis during nitrogen starvation. Bioresources and Bioprocessing, 10(1), 70. https://doi.org/10.1186/s40643-023-00700-0
Santos, B., da Conceição, D. P., Corrêa, D. O., et al. (2022). Changes in gene expression and biochemical composition of Haematococcus pluvialis grown under different light colors. Journal of Applied Phycology, 34, 729–743. https://doi.org/10.1007/s10811-022-02696-0
Scibilia, L., Girolomoni, L., Berteotti, S., Alboresi, A., & Ballottari, M. (2015). Photosynthetic response to nitrogen starvation and high light in Haematococcus pluvialis. Algal Research, 12, 170–181. https://doi.org/10.1016/j.algal.2015.08.024
Silva, D. L., de Moraes, L. B. S., Oliveira, C. Y. B., da Silva Campos, C. V. F., de Souza Bezerra, R., & Gálvez, A. O. (2022). Influence of culture medium on growth and protein production by Haematococcus pluvialis. Acta Scientiarum. Technology, 44, e59590. https://doi.org/10.4025/actascitechnol.v44i1.59590
Sun, H., Liu, B., Lu, X., Cheng, K. W., & Chen, F. (2017). Staged cultivation enhances biomass accumulation in the green growth phase of Haematococcus pluvialis. Bioresource Technology, 233, 326–331. https://doi.org/10.1016/j.biortech.2017.03.011
Tocquin, P., Fratamico, A., & Franck, F. (2012). Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of a low N/P ratio on vegetative growth. Journal of Applied Phycology, 24(3), 365–373. https://doi.org/10.1007/s10811-011-9771-3
Tran, H. L., Lee, K. H., & Hong, C. H. (2015). Efectos de la irradiación con LED en el crecimiento y la producción de astaxantina de Haematococcus lacustris. Biosciences Biotechnology Research Asia, 12(2), 1153–1159. https://doi.org/10.13005/bbra/1778
Wang, Q., Wang, H., Ning, Y., Luo, Q., Yang, X., Huang, Y., et al. (2021). Transcription factors from Haematococcus pluvialis involved in the regulation of astaxanthin biosynthesis under high light–sodium acetate stress. Frontiers in Bioengineering and Biotechnology, 9, 2021. https://doi.org/10.3389/fbioe.2021.650178
Wang, J., Sommerfeld, M. R., Lu, C., & Hu, Q. (2013). Combined effect of initial biomass density and nitrogen concentration on growth and astaxanthin production of Haematococcus pluvialis (Chlorophyta) in outdoor cultivation. Algae, 28(2), 193–202. https://doi.org/10.4490/algae.2013.28.2.193
Wei, Z., Sun, F., Meng, C., Xing, W., Zhu, X., Wang, C., et al. (2022). Transcriptome analysis of the accumulation of astaxanthin in Haematococcus pluvialis treated with white and blue lights as well as salicylic acid. BioMed Research International, 2022, 4827595. https://doi.org/10.1155/2022/4827595
Xi, T., Kim, D. G., Roh, W., Choi, J. S., & Choi, Y. E. (2016). Enhancement of astaxanthin production using Haematococcus pluvialis with novel LED wavelength shift strategy. Applied Microbiology and Biotechnology, 100, 6231–6238. https://doi.org/10.1007/s00253-016-7301-6
Zhang, L., Su, F., Zhang, C., Gong, F., & Liu, J. (2016). Changes of photosynthetic behaviors and photoprotection during cell transformation and astaxanthin accumulation in Haematococcus pluvialis grown outdoors in tubular photobioreactors. International Journal of Molecular Sciences, 18(1), 33. https://doi.org/10.3390/ijms18010033
Zhang, W., Zhou, X., Zhang, Y., Cheng, P., Ma, R., Cheng, W., et al. (2018). Enhancing astaxanthin accumulation in Haematococcus pluvialis by coupLED light intensity and nitrogen starvation in column photobioreactors. Journal of Microbiology and Biotechnology, 28(12), 2019–2028. https://doi.org/10.4014/jmb.1807.07008
Zhao, Y., Yue, C., Geng, S., Ning, D., Ma, T., & Yu, X. (2019). Role of media composition in biomass and astaxanthin production of Haematococcus pluvialis under two-stage cultivation. Bioprocess and Biosystems Engineering, 42, 1–3. https://doi.org/10.1007/s00449-018-02064-8
Descargas
Los datos de descargas todavía no están disponibles.
Datos de publicación
Metric
Este artículo
Otros artículos
Revisores/as por pares
0
2.4
Perfil evaluadores/as N/D
Declaraciones de autoría
Declaraciones de autoría
Este artículo
Otros artículos
Disponibilidad de datos
N/D
16%
Financiación externa
No
32%
Conflictos de intereses
N/D
11%
Metric
Esta revista
Otras revistas
Artículos aceptados
2%
33%
Días para la publicación
0
145
- Editor y equipo editorial
- Perfiles
- Sociedad académica
- Universidad de Bogotá Jorge Tadeo Lozano
- Editorial
- Universidad de Bogotá Jorge Tadeo Lozano
Para obtener más información sobre un dato, haga clic 
Public Facts Label - Plugin Mantenido por Public Knowledge Project
